anti human cd45 alexa fluor 647  (Bio-Rad)

Journal: Oncogene

Article Title: Polyploid cancer cells reveal signatures of chemotherapy resistance.

doi: 10.1038/s41388-024-03212-z

Figure Lengend Snippet: Fig. 1 Large tumor cells are found in bone marrow aspirate of late-stage prostate cancer patients. A Percentage of patients with matched peripheral blood and bone marrow samples with at least 1 CTC-IGC present in liquid biopsy. B Representative images of a typical CTC (left) and CTC-IGCs (middle and right) found in bone marrow aspirate. Far right image depicts two (n = 2) CTC-IGC cells that are EPI-negative but are confirmed to be tumor derived (see Fig S2A). Scale bars set to 15 µM. C PFS from patients with (teal) or without (red) at least one CTC-IGC found in bone marrow samples. D Representative image of typical CTC found in bone marrow with merged and DAPI channels (top) and its genomic copy number profile (bottom). E Representative image of CTC-IGC found in bone marrow with merged and DAPI images (top) and its genomic copy number profile (bottom). F Representative image of mono-nucleated CTC-IGC found in bone marrow with merged (pan- epithelial markers in red, CD45 in green, vimentin in white, and DAPI in blue) and DAPI images (top) and its genomic copy number profile (bottom). Scale bars are set to 15 µM in D–F.

Article Snippet: Patient slides in Fig. 1 were fixed with paraformaldehyde and stained with a pan-cytokeratin cocktail mixture (see supplementary methods), conjugated mouse anti-human CD45 Alexa Fluor 647 (clone: F10-89-4, MCA87A647, AbD Serotec, Raleigh, NC, USA), Vimentin (Alexa Fluor 488 rabbit IgG monoclonal antibody (Cell Signaling Technology; Cat# 9854BC; Clone: D21H), and 4’,6- diamidino-2-phenylindole (DAPI; D1306, Thermo, Waltham, MA, USA) as previously described [46].

Techniques: Derivative Assay